摘要
目的 了解我国HIV感染者p2 4蛋白编码区的基因变异情况。 方法 收集我国河南及上海地区 2 8份已经证实的HIV - 1感染患者的血浆标本并抽提出RNA ;采用逆转录和Nested PCR的方法扩增所需的目的基因 ,使用DNA测序仪直接测序 ;应用CLUSTALW、PHYLIP等软件包对序列进行比对及进化树分析。结果 2 8份gag样本中 2 5份为B亚型 ,3份为A亚型。与共享序列相比 ,2 5例B亚型的 p2 4编码区中发生核苷酸改变的位点比例为 0 .4 %～ 4 .8% ,平均为 1.4 % ,其中A→G占 2 0 .5 % ,G→A占 17.3% ;3例A亚型发生核苷酸改变的位点比例平均为 0 .2 4 %。在所有变异位点中均未发现G→A的高度突变。B亚型和A亚型内的基因离散率平均为 2 .9%和 0 .5 8% ,A亚型与B亚型之间的基因离散率平均为 11.1%。 2 5例B亚型根据基因序列所推测的p2 4蛋白发生氨基酸改变的比例为 0 .4 %～ 5 .2 % ,平均为 2 .2 %。进化树分析表明在我国河南地区HIV感染B亚型多为与泰国株相近的B’亚型。结论 HIV 1p2 4编码区仍相对保守 。
Purpose: To comprehend the variation of p24 coding region in gag gene of HIV-1 in China. Methods: Plasma specimens were collected from 28 HIV-1 patients in Henan province and Shanghai. RNAs were extracted and the target gene was amplified by the method of RT and Nested-PCR. The 693 nucleotide-sequence of p24 region was determined. Phylogenetic analyses were performed. Results: Of 28 specimens, 25 were of B subtype and 3 were of A subtype. Comparing to the consensus sequence, nucleotide variation in B subtype was 0.4 % - 4.8 %, with an average of 1.4 %. The transition changes of A-to-G and G-to-A were 20.5 % and 17.3% respectively. While the G-to-A hypermutation was not observed. The intrasubtype distance for B subtype was 2.9%, and the substitution for predicted amino acid was 0.4% - 5.2%. Because of the small number, the variation in A subtype was much lower than that of B subtype. Phylogenetic analyses implied that most of HIV-1 B subtype infections in Henan province were close to Thailand isolate. Conclusions: The changes of p24 coding region of HIV-1 in Chinese patients in this study was still relatively conservative. Target at the conserved regions would be beneficial to the research of immunotherapy and vaccine.
出处
《复旦学报（医学版）》
EI
CAS
CSCD
北大核心
2004年第4期371-374,共4页
Fudan University Journal of Medical Sciences
基金
教育部归国留学人员科研启动基金(教育部 66)资助项目
