摘要
利用改进了的ＤＮＡ提取方法，从“三系”杂交水稻汕优６３的不育系、保持系、杂种一代和恢复系单株中提取ＤＮＡ，进行特异基因片断的ＰＣＲ基因扩增分析。结果表明：从２００个随机引物和设计的特定引物中筛选出３个引物，能有效地从不育系中鉴别出保持系，从汕优６３中分别鉴定出不育系、保持系、恢复系。研究结果表明，利用这些ＤＮＡ分子标记鉴定种子纯度，具有快速、准确、可靠的优点。
The PCR analysis for the seed Durity
detection of hybrid rice“Shanvou 63” isreportcd in this paper.By the use of motified CTAB
method,the DNA samples from riccseedlings were extracted effectively.From 200 random
primers and 7 special primers.three primers PFA,PFB,PAB,were selected for seed purity
detection.By using PFAaid PFB,we can identify the male sterile line(Zhen-Shan 97A)and
restore line(MingHui 63)seeds from hybrid seeds(Shan-You 63);and by using PAB we can
distinguish themale sterile line and maintainer line seeds (Zhen-Shan 97B)from each otber.The
resultsindicate that PCR analysis is an effective,accutive and simple way for seed purity
detec-tion.
出处
《西南农业大学学报（自然科学版）》
CSCD
1996年第2期148-151,共4页
Journal of Southwest Agricultural University
