摘要
目的研究肾上腺髓质素(ADM)及其特异性受体-受体活性调节蛋白2/降钙素受体样受体(RAMP2/CRLR)mRNA在正常肾上腺髓质和嗜铬细胞瘤组织中的表达。方法提取8例正常肾上腺髓质和18例嗜铬细胞瘤组织总RNA,行逆转录-聚合酶链反应,以扩增的ADM、RAMP2、CRLR条带灰度与内参照GAPDH条带灰度比值进行半定量分析。结果正常肾上腺髓质和嗜铬细胞瘤组织中均可见ADM及其特异性受体RAMP2/CRLRmRNA的表达,其ADM/GAPDH分别为0.48±0.09和0.75±0.24,RAMP2/GAPDH分别为0.79±0.12和1.29±0.30,CRLR/GAPDH分别为0.40±0.08和0.87±0.22,嗜铬细胞瘤组织中ADM及其特异性受体RAMP2/CRLRmRNA的表达显著高于正常肾上腺髓质(P<0.05)。结论ADM可能通过自分泌或旁分泌方式作用于肾上腺局部,在嗜铬细胞瘤的发生发展中起一定的作用。
Objective To investigate the expression of human adrenomedullin （ADM） and its receptorreceptor activity modifying protein 2/calcitonin receptor-like receptor （RAMP2/CRLR） mRNA in the tissues of normal adrenal medulla and pheochromocytoma. Methods Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA. Results Expressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48±0.09 and 0.75±0.24, RAMP2/ GAPDH 0.79 ± 0.12 and 1.29 ± 0.30, CRLR/GAPDH 0.40 ± 0.08 and 0.87 ± 0.22 in normal adrenal medulla and pheochromocytomas, respectively （P 〈 0.05）. Conclusion ADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2005年第4期452-456,共5页
Acta Academiae Medicinae Sinicae
基金
首都医学发展科研基金(ZD199908)
卫生部科技专项课题(WKZ-2000-1-36)~~
