摘要
在本项研究中,应用反相C18固定相超临界CO2色谱对胡萝卜素、叶黄素和叶绿素的分离进行了初步的探索。胡萝卜素、叶黄素和叶绿素的下述色谱条件下被完全分开:色谱柱:ZorbaxODS;流动相:3%(V/V)正丙醇及超临界CO2流体;超临界条件:压力:4000psi;温度:40℃;流速:2.0ml/min;检测波长:435nm。与反相C18高压液相色谱结果相比,这些组分的超临界色谱呈正相洗脱行为。这一结果表明:超临界CO2流体具有很强的非极性,同时,也证明了预装的反相C18柱可以作为正相固定相来分离这些化合物.
A primary investigation into the separation of carotene, xanthophylls and chlorophylls on reversed-phase C18 supercritical carbon dioxide chromatography was made in this study. Carotene, xanthophylls and chlorophylls were readily separated under the conditions as follow： Stationary phase： Zorbax ODS; Mobile phase： 3% （V/V） propanol-1 in CO2; S upercitical conditions： pressure： 4000psi; temperature： 40℃; flow rate： 2.0ml/min; detection： 435nm. In comparison with the chromatographic profit of those fractions on reversed-phase C18 HPLC, their SFC profit showed a normal phase pattern. This observation suggested that supercritical carbon dioxide was strong apolar and pre-packed reversed phase C18 column could be employed as normal phase stationarv phase for the separation of those compounds.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第8期162-166,共5页
Food Science
