摘要
目的:用双向电泳分析牙髓细胞分化过程中产生或分泌的新蛋白质.方法:在体外培养牙髓细胞,给予一定时间的1,25-双羟维生素D3,促使牙髓细胞分化,结合双向电泳技术分析细胞分化前后蛋白质合成、分泌的变化.结果:pH4-8 和相对分子量MW 20～90 kD范围的蛋白斑点分布较多.双向电泳凝胶图像分析软件ImageMaster 2D Platium比较分析,给予维生素D孵育24 h后,检测到77个新的蛋白点,同时有17个蛋白点消失.结论:维生素D促使牙髓细胞分化,并产生或分泌了一些新的蛋白质,维生素D和这些蛋白质对牙齿的发育、矿化的过程起调节作用.
Objective： The purpose of this paper is to study new proteins produced or secreated by rat dental pulp cells in the course of development. Methods： Isolated of rat dental pulp cells were cultured in vitro. Two groups were incubated with vitamin D for 3h and 24h respectively and one group was set as control. Two - dimensional electrophoresis was used to analysis the proteins of cell culture supernatant. Results： The differential protein expression analysis found that 77 protein spots were specifically detected, and 17 protein spots disappeared in the group treated with Vit D for 24 h. Most spots were focused in the area of pH4 - 8 and 20 - 90 kD. Conclusion ： It is concluded that Vit D may play a role in the development of rat dental pulp cells, which produce some new proteins certified by 2 - D electrophoresis. It is suggested that some new proteins have been produced or secreted by rat dental pulp cells in the course of development.
出处
《口腔医学研究》
CAS
CSCD
2005年第4期402-404,共3页
Journal of Oral Science Research
关键词
双向电泳
维生素D
牙髓细胞
发育
2-D eleetrophoresis Vitamin D Dental pulp cells Development
