摘要
目的采用乳液冷冻干燥法制备用于组织修复的明胶多孔支架材料,考察主要制备参数对支架材料性能的影响,为进一步制备满足细胞培养和临床应用的支架材料提供依据。方法采用乳液冷冻干燥法,将明胶溶液与有机醇混合制成乳液,预冻,冷冻干燥得到支架材料,考察其性能。结果制备过程中,乳液的固含量,预冻温度对支架材料的微观结构、表观密度、含水量和孔隙率有较大影响。结论采用乳液冷冻干燥法可以成功的制备明胶多孔支架材料,通过控制反应条件和参数可以调整支架孔隙结构和性能,从而得到满足需要的支架材料。
Objectives To prepare porous gelatin scaffolds by emulsion freeze-drying method for tissue repair, to investigate the influence of the main preparation parameters on scaffold properties and provide the basis for preparation of the scaffolds used for further cell culture and clinical application. Methods The gelatin solutions were mixed with pentanol at certain volume ratios to obtain stable emulsion. The emulsion was frozen, freeze-dried and then treated using ethyl alcohol to get scaffolds. The microstructure of the scaffolds was observed by scanning electron microscopy (SEM) and the properties were characterized by the apparent density, water uptake ability and porosity. Results The gelatin scaffolds had interconnected pore structure with average pore diameter of 15μm～40μm and porosity larger than 90%. The solid content of emulsion and the pre-freezing temperature affected the microstructure, apparent density, water uptake ability and porosity of scaffolds. Conclusion Porous gelatin scaffolds can be successfully prepared by emulsion freeze-drying method. By controlling the reaction conditions, the pore structure and properties can be adjusted to satisfy the needs.
出处
《组织工程与重建外科杂志》
2006年第2期92-95,共3页
Journal of Tissue Engineering and Reconstructive Surgery
