摘要
将ＡＥＶ－ＶＲ株接种于６日龄ＳＰＦ鸡胚卵黄囊，孵化至１６日龄时迫杀，采集鸡胚绒毛尿囊膜、尿囊液、胚体称重，研磨、离心取上清液加入福尔马林灭活，以灭菌ＰＢＳ（ｐＨ７．２）稀释成１：１、１：１０、１：２０三种浓度，加入吐温－８０制成水相，另以白油、司斑－８０和硬质酸铝煮沸灭菌制成油相，搅拌混合制成不同浓度的灭活油乳剂疫苗，分别免疫２日龄ＳＰＦ雏鸡，安全可靠．用ＡＥＶ－ＶＲ、ＡＥＶ－１１４３、ＡＥＶ－ＮＨ９３７三株混合毒做攻毒试验证明１：１稀释的灭活苗效果最好，保护率可达１００％．用ＡＥＶ－ＶＲ，ＡＥＶ－１１４３、ＡＥＶ－ＮＨ９３７及以上三株混合毒同法制成１：１浓度的灭活油乳剂疫苗，分别免疫２日龄商品雏鸡后用其相应的毒株做攻毒试验，表明该疫苗是安全的，而且可使免疫鸡抵抗ＡＥＶ的感染，具有良好的免疫效果．
Abstract day old-SPF embryos were inoculated with Avisn Encephalomyelitis Virus(AEV) Van Roekel strain, 1143 train and NH-937 Strain via yoolk sac and killed 10 days podinfection. Then 1 : 1, 1:10, 1: 20(w/v)homogenates of allantois,allantoic fluid and embryos bodies prepered in PH7. 2 PBS.After inactivated with formalin,fhe oil emulsion vaccine was produced by miking the homgenate with tween-80,liquid paraffin,span-80 and aluminium stearate. Chicks of 2 day old were vaccinated by intramuscular inoculation. Thees vaccinates were challenged with AEV Van Roekel strain,1143 sttain. or NH-937 strain 20 days postinoculation. The results showed that the 1:1 (w/v) of inactiveated oil emulsion vaccine against AE was safe and effective.
基金
国家自然科学基金
