摘要
采用ＮＤＬａｓｏｔａ株为种毒，福尔马林为灭活剂，７号白油为佐剂，吐温８０、司本８０为乳化剂，硫柳汞为防腐剂，高压匀浆泵为乳化工具，研制出ＮＤ油性剂灭活疫苗。其剂型为双相型（Ｗ／Ｏ／Ｗ），呈稀薄乳状液，粒度为１μｍ左右，粘度≤１ｓ，通针性好。４～８℃有效保存期１年。效力≥７０ＰＤ５０，最早产生免疫时间为１２天（１００％保护），生产中常用免疫剂量为２月龄内每只０．５ｍｌ，２月龄以上每只１．０ｍｌ皮下注射，对各日龄鸡安全无副作用。与ＮＤ活毒疫苗同时免疫可使各日龄鸡产生高效价的ＨＩ抗体，雏鸡免疫期为２．５～３个月，成年鸡为１２个月。此苗可有效地预防强毒力嗜内脏型ＮＤ及“非典型”ＮＤ。
Inactivated oil emulsion vaccine against ND was studied and prepared successfully,by using NDV Lasota strain as seed virus,formalin as inactivating agent,No 7 white mineral oil as adjuvant, Tween-80 and span-80 as emulsifiers, merthiolate as preservative. This oil-emulsion vaccine, of a double emulsion type (W/O/W), had particles about 1μm in diameter. The vaccine was thin emulsion with the viscosity≤1 second,it was easy to inject. Under 4--8℃,the vaccine can be preserved for over one year. Potency tests indicated the dosage was ≥70PD50 The earliest immunity was produced at 12 days after inoculation (100% prortection ). Practically, the preferred dosage was 0. 5ml per bird for birds within 2 months of age, and 1. 0ml per bird for the birds older than 2 months of age. The vaccine was safe for chickens of any ages, and had no adverse effects. When combined with live ND vaccines, it could induce high HI antibody for chicks of any ages. Immune periods lasted 2. 5 to 3 months for chickens and more than 6 months for adult birds. The viscerotropic velogenic ND and atypical ND can be prevented effectively by using this vaccine.
出处
《华北农学报》
CSCD
北大核心
1995年第3期115-123,共9页
Acta Agriculturae Boreali-Sinica
关键词
新城疫
灭活疫苗
油佐剂
疫苗
鸡病
Newcastle Disease (ND)
Inactivated vaccine
Oil Emulsion
