摘要
采用间隔２４ｈ静脉注射内毒素（ＬＰＳ）的方法，给家兔诱导全身性施瓦茨曼反应（ＧＳＲ），以此作为家兔内毒素性播散性血管内凝血（ＤＩＣ）模型。在造模前后不同时间采用ＥＬＩＳＡ方法测定血浆肿瘤坏死因子（ＴＮＦ）水平。结果：在第１次注射ＬＰＳ后２ｈ血浆ＴＮＦ水平最高（７６．１８±２３．１８ｕｇ／Ｌ），其次为第２次注射ＬＰＳ后６ｈ（６６．９７±２４．７５ｕｇ／Ｌ）和１２ｈ（５７．９０±２１．５０ｕｇ／Ｌ，均较实验前（２１．６０±５．９９ｕｇ／Ｌ明显升高（Ｐ＜０．０１）。作者初步讨论了不同时相出现ＴＮＦ高峰的可能机制。
Animal mddel of disseminated intravenous coagula-tion(DIC)
was made in rabbits by injecting endotoxintwice at 24 hours interval.We measured the plasma
levels of tumor necrosis facter(TNF)at defferent ime byusing ELISA and found the TNF level 2
hours after thefirst injection of endotoxin was the highest(76.18±23.18 ug/L).The TNF levels 6
and 12 hours after thesecond injection of endotoxin 66.97±24.75 ug/L and57.90 ±21.50 ug/L.
respeetivel which were all higherthan that before experiment(2l.60±5.99 ug/L ) Thepossible
mechanism of the peak TNF that appeared atdefferent time was discussed
出处
《中国危重病急救医学》
CAS
CSCD
1995年第2期65-67,共3页
Chinese Critical Care Medicine
关键词
播散性
血管内凝血
内毒素性
肿瘤坏死因子
dissminated intravascular coagulation
endotoxin,tumor necrosis facter (TNF)
