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单克隆抗体-ELISA技术检测间日疟感染的现场研究 被引量:6

A FIELD STUDY ON MCABELISA TECHNIQUE IN DETECTION OF BLOOD-STAGE PLASMODIUM VIVAX ANTIOEN
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摘要 对112例发热病人血样同时进行镜检和用McAb-McAbELISA夹心法检测间日疟抗原,发热病人血片分别由基层镜检员和省级专业人员镜检,检出间日疟阳性率分别为30.36%和43.75%,ELISA法检测阳性率为42.86%,其敏感性、特异性均在95%以上。具有采样方便、样本可成批操作、结果客观等特点,适用于基层开展灭疟后期疟疾传染源监测。 McAb-McAb sandwich ELISA was used to detect Plasmodium vivax antigen inwhole blood of 112 blood samples of feverish patients collected from malarious area in NorthernFujian , while the feverish films were examined both by local personnels and provincial specialistin which the positive rate ofP. vivax were 30. 36%and 43.75%respectively. The positive rate ofELISA detection was 42. 86%with the sensitivity and specificity both being above 95%. The re-sults showed that the assay had the characteristics of convenient sample collection,mass opera-tion ,objective output , and suitibility to use in malaria surveillance.
出处 《中国寄生虫病防治杂志》 CSCD 1995年第3期200-201,共2页 Chinese Journal of Parasitic Disease Control
基金 福建省自然科学基金
关键词 单克隆抗体 ELISA 间日疟 疟疾 Monoclonal antibody ,ELISA,Plasmodium vivax
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  • 1张山鹰,黄柏芳,许龙善,李莉莎,吴金俊,林宗琳,何秀华.单克隆抗体-ELISA技术检测间日疟红内期抗原的初步研究[J]中国人兽共患病杂志,1992(01). 被引量:1

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