摘要
将小鼠腹腔巨噬细胞和含载脂蛋白Ｂ的两种脂蛋白、这两种脂蛋白与硫酸葡聚糖或相应抗体形成的复合物一起培养２４ｈ后，用薄层层析法测定巨噬细胞内胆固醇酯的含量。结果发现，天然脂蛋白（ａ）对巨噬细胞内胆固醇酯含量影响不大（增加８％，Ｐ＞０．０５）；天然低密度脂蛋白具有促进巨噬细胞内脂质蓄积作用（增加３５．４％，Ｐ＜０．０５）；上述两种脂蛋白与硫酸葡聚糖一起共同作用，促进巨噬细胞内胆固醇酯蓄积作用明显加强（分别由增加８％和３５．４％提高到增加６８．１％和７７．０％，Ｐ＜０．０１）；将硫酸葡聚糖改为抗载脂蛋白（３）或Ｂ抗体，再共同培养２４ｈ，上述两种脂蛋白的这种加强巨噬细胞内胆固醇酯蓄积的作用更加明显（分别提高到增加１５４．０％、１６１．９％和１７６．１％，Ｐ＜０．００１）。以上实验结果表明，含载脂蛋白Ｂ的脂蛋白与硫酸葡聚糖或相应抗体的复合物能促进巨噬细胞内脂质的蓄积。
Macrophages were incubated with apolipoprotein B-containing lipoproteins, the complexes of lipoprotein with dextran sulfate (DS) or antibodies (lipoprotein cholesterol 200 mg·L￣(-1), dextran sulfate 0.4 mg or antibodies 0.1 ml) for 24 h. The cholesteryl esters (CE) in macrophages were determined by thinlayer chromatography. The results that native lipoprotein (a) did not give rise to any significant storage of intracellular cholesteryl esters (0. 9 mg·g￣(-1) cell protein,P>0. 05); low density lipoprotein (LDL)increased CE formation in macrophages ( 4.0 mg·g￣(-1) cell protein, P<0. 05); lipoprotein (a)-DS or LDL-DS complexes caused a significant enhance in CE formation in macrophages, which were 7. 7 ,and 8. 7 mg·g￣(-1) cell protein ,respectively (P < 0. 01 ); similar result were observed when macrophages were incubated with both the complexes of lipoprotein (a) with antibodies against apolipoprotein (a) or apolipoprotein B and LDL with antibodies against apolipoprotein B,which were 19.9, 18.3 and 17. 4 mg·g￣(-1) cell protein, respectively (P<0. 01 ) . The above data showed that the complexes of apolipoprotein B-containing lipoproteins with DS or antibodies could cause lipid accumulation in macrophages.
出处
《中国动脉硬化杂志》
CAS
CSCD
1995年第3期240-243,共4页
Chinese Journal of Arteriosclerosis
关键词
动脉粥样硬化
载脂蛋白B
脂蛋白
低密度脂蛋白
Lipoprotein(a)
Low density lipoprotein
Macrophage
Dextran sulfate
Antibody
