摘要
实验用ＳＤ大鼠。ＣＣｌ４经口染毒５００ｍｇ／ｋｇ。每周２次。连续染毒６周。染毒的同周分别给予锌（６ｍｇ／ｋｇ混入饲料中喂饲）。硒（３μｇ／ｍｌ饮用水供饮用）及ＶｉｔＥ（１０ｍｇ／ｋｇ腹腔注射，每周二次），共给１０周。于每隔两周处死一批动物，１０周实验的结果显示，ＣＣｌ４染毒后表现为ＳＧＰＴ活性、ＭＤＡ含量升高，ＧＳＨ、Ｐ４５０及ｂ５的含量下降，Ｚｎ、Ｓｅ和ＶｉｔＥ处理组其ＳＧＰＴ活性升高的幅度低于ＣＣｌ４组，上升的峰值也迟两周出现。停止染毒后，Ｚｎ，Ｓｅ组ＳＯＰＴ活性迅速下降，于第八周恢复对照组水平，并比ＶｉｔＥ组早两周达正常。各处理组ＭＤＡ含量值也表现为上升的幅度低，染毒停止后迅速恢复。Ｚｎ组ＧＳＨ第六周才有显著下降，第八周即恢复，Ｓｅ组则在整个观察期未见有显著的下降。各组染毒后细胞色素Ｐ４５０含量均下降。除Ｚｎ组于第八周达对照组水平外，其他组至第一周仍低于阴性组，细胞色素ｂ５含量Ｚｎ组、Ｓｅ组和ＶｉｔＥ组则于第四周开始下降，第八周恢复。组织病理学改变表现为ＶｉｔＥ、Ｚｎ、Ｓｅ组肝损害程度轻于ＣＣｌ４组。在抗纤维化上Ｓｅ和Ｚｎ优于ＶｉｔＥ。
In the experiments the SD rats were chosen and were given per os at a dose of 500 mg/kg for six weeks,two times per week.Zn-containing diet at the dose of 6 mg Zn/kg ,Se-containing water at a dose of 3μg/ml sodium selenite anhydrous ,VitE were given per os by peritoneal injection at the dose 10mg/kg ,two times per week.All administraton had started at the same time with the CCl4 treatment but continued,ten weeks.The results showed that the CCl4 treatment elicited the increase of MDA content and SGPT activity and the decrease of the GSH ,cytochrome P450 and b5. Zn ,Se and VitE played a protective role as :The increasing degree of SGPT and MDA was lower than that of the CCl4 group,and it recovered soon after ending the CCl4 treatment.GSH quantity decreased in the sixth week in Zn group ,while no remarkable chanses in Se group at whole experiment period Cytochrome P450 content decreased remarkable after CCl4 treatment and Iasted ten weeks besides that in Zn group recovered in tenth weeks Also,the b5 decrease were found by the CCl4 treatment ,but the recovered in Zn,Se and Vit E group was found in the eighth week Pathology results showed that the Zn ,Se and Vit E might play a protective role on the liver injury caused by CCl4 ,in which the anti-fibrosis action of Zn and Se seems to be better than that of VitE.
出处
《卫生毒理学杂志》
CSCD
1995年第2期80-83,117,共5页
Journal of Health Toxicology
基金
美国中华医学基金
关键词
锌
硒
四氯化碳
保护作用
肝损害
药理学
Carbon tetrachloride
Liver injury
Protective role
zinc
Seleniuml
Vitamin E
