摘要
将两株杂色曲霉菌分别接种于4kg玉米豆粉固体产毒培养基,28℃静置培养35d。以甲醇-4％ KC1(9:1)混合液及甲醇-三氯甲烷混合液浸提杂色曲霉素(sterigmatocy stin,简称ST),再用三氯甲烷萃取,经硅胶柱层析法初步纯化。在甲醇、乙腈、乙醚、丙酮等溶剂中多次重结晶,最后得到2271.6mg淡黄色针状结晶。经过熔点测定、元素分析、四种光谱学鉴定、薄层层析及高效液相色谱鉴定,确认该晶体为ST。纯度在99.9％以上。
Two strains of Aspergillus versicolor producing ST at 550.8mg·kg-1 substrate and 1160.8mg·kg-1 substrate were selected to inoculate 4 kg solid ST-pro-ducing media. After 35 days stationary incubation at 28℃ in the dark, 2271.6mg of pale-yellow needle-shaped crystals were isolated and purified from the culture with a procedure applying column chromatography and recrvstallization method. The crystal was proved to be sterigmatocystin by spectroanalysis and some physico-chemical analysis. The purity of the final material obtained were more than 99.9% as shown by HPLC and TLC detection. With this procedure, ST was obtained at about one tenth of its commercial cost.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第5期358-363,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金资助课题
