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杂色曲霉素高产菌株的筛选 被引量:2

Screening of sterigmatocystin-producing aspergillus strains
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摘要 用薄层层析法初步筛选16株曲霉在5种培养基上产生杂色曲霉素(ST)的能力后,选定14株比较它们在玉米豆粉固体培养基上的ST产量。高压液相色谱测定发现,经28℃一个月静置培养后,ST最高产量达1.16g/kg,比国内文献报道产量高4~23倍。高产菌株已用于大量制备ST。 Fourteen strains of Aspergillus versicolor and 2 strains of Aspergillus nidulans were screened for their sterigmatocystin-producing ability on 5 different complex substrates by TLC and HPLC. Fourteen of them can produce sterigmatocystin(ST)on all the substrates, while 1 from each group was negative.The highest yield was found on a corn-soybean mixed solid medium by a strain named AVi.After 30 days stationary incu- bation at 28℃,AV1 produced ST at a level of 1160.8μg/g.This is 4 to 23 times higher than those reported in China.
出处 《卫生研究》 CAS CSCD 北大核心 1993年第5期299-302,共4页 Journal of Hygiene Research
基金 国家自然科学基金
关键词 杂色曲霉素 产生菌 高效液相色谱 sterigmatocystin, aspergillus, TLC, HPLC
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