摘要
交链孢醇单甲醚是从林县污染粮食中分离的互隔交链孢霉的提取物,经进一步纯化结晶获得,它是该霉菌的主要代谢产物之一。本文用V_(79)细胞突变的方法进行了交链孢醇单甲醚的致突变性研究。结果表明,在不加S-9和加S-9情况下,交链孢醇单甲醚均能诱发V_(79)细胞6-巯基鸟嘌呤抗性突变,随交链孢醇单甲醚剂量增大,突变频率增高,具有直线回归关系(P<0.01),但不加S-9时比加S-9时致突变作用更强,提示交链孢醇单甲醚是直接致突变物,可能与食管癌病因有一定关系。
Alternariol monomethyl ether(AME) was isolated, purified and crystallized from the extracts of the corn inoculated with Alternaria alternata which was separated from the contaminated rice in the high incidence area of esophageal cancer, Lixian County. AME is one of the major metabolic products of A. alternata. In this paper, the mutagenic potency of AME was detected with the V_(79) Chinese hamster cells. The experimental results showed that with or without rat liver microsomes (S-9), AME could induce 6-Thioguanine resistant (6-TG^r) mutation in V_(79) cells and their dose-effect curves were similar. The mutagenicity of AME without S-9 was stronger than that of AME with S-9. So it is considered that AME is a direct mutagen which doesn' t need to be activited metabolically by S-9. AME may play an important role in the etiology of esophageal cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1989年第3期154-157,共4页
Chinese Journal of Pathophysiology
关键词
食管肿瘤
交链孢霉
诱变试验
Alternaria
Mutagenicity tests
Esophageal neoplasms
