摘要
目的:观察整体大鼠胃酸分泌与ATP水平之间的关系及迷走神经对解偶联蛋白2(UCP2)mRNA表达的调节。方法:制备大鼠高选择性迷走神经切断模型。采用滴定法检测大鼠胃酸酸度,荧光测定法检测胃体组织内ATP含量,应用Northernblot法检测分析UCP2mRNA表达。结果:迷走神经切断后24h,大鼠胃酸酸度显著降低,胃体组织内ATP含量下降。与假手术组相比,胃体组织UCP2mRNA表达显著增加。结论:迷走神经切断后24h大鼠胃体组织ATP含量下降;迷走神经下调胃体组织UCP2mRNA表达。结果提示大鼠迷走神经可能通过抑制UCP2mRNA表达,提高ATP含量,为质子泵泌酸提供能量来源。
Aim： The relationship between gastric acid secretion and ATP level, and regulation of uncoupling protein2（UCP2） mRNA expression by vagus nerve were studied in vagotomied rats. Methods： With the high selective vagotomy model, the gastric acidity was titrated to pH 7.0 with 0.1 mol/L NaOH solution and ATP contents were quantified by using fluoremetry. The expression of UCP2 mRNA was observed by using Northern blot in stomach of rats. Results： Both of gastric acidity and ATP contents in stomach body decreased significantly at 24 h after vagotomy. The expression of UCP2 mRNA was markedly increased as compared with sham operation group. Conclusion： ATP contents decreased and vagus nerve down-regulates expression of UCP2 mRNA in stomach corpus in vagotomied rats. The results indicates that vagus nerve could underlay the gastric acidity by inhibiting expression of UCP2 mRNA and increasing ATP contents in rats
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2005年第3期290-292,共3页
Chinese Journal of Applied Physiology
基金
教育部留学回国人员科研启动基金资助(200314)
辽宁省教育厅科技攻关计划项目(202203243)
