摘要
目的研究乳腺癌组织中环氧化酶2(COX2)的表达及其与VEGF、MVD、MMP9和TIMP1的相互关系,探讨其在乳腺癌发生和浸润中的生物学意义。方法应用SP法检测65例浸润性乳腺癌组织中COX2、VEGF、MMP9、TIMP1及血管内皮标记物CD34的表达情况,并观察7例导管原位癌(DCIS)、45例癌旁乳腺组织中COX2的表达。结果65例浸润性乳腺癌、7例DCIS均表达COX2,其中浸润性乳腺癌中COX2高表达率为66.2%(43/65),而45例癌旁组织中21例有COX2弱表达,两者之间差异有显著性(P<0.01)。COX2与乳腺癌患者的年龄、肿瘤大小、组织学分级、TNM分期、腋窝淋巴结转移均无相关性,但COX2在浸润性乳腺癌中的表达与VEGF、MMP9、TIIMP1及MVD均呈正相关(P<0.05)。结论COX2可能与乳腺癌组织中微血管形成和细胞外基质代谢有关。
Purpose To study the expression of cyclooxygenase-2 （COX-2） in breast cancer, and to explore the relationship of COX-2 expression with matrix metalloproteinase-9 （ MMP-9 ）, tissue inhibitor of matrix metalloproteinase-1 （ TIMP-1 ）, vascular endothelial growth factor （VEGF） and microvessel density （MVD）. Methods The expression of COX-2, VEGF, CD34, MMP-9, TIMP-1 in 65 cases of invasive breast carcinoma, and COX-2 expression in 7 cases of ductal carcinoma in situ （DCIS） and 45 cases of paired breast tissues were detected by the method of S-P immunohistochemical staining. Results COX-2 was expressed in all 65 cases of invasive breast carcinoma and 7 cases of DCIS, in invasive carcinomas the strong positive rate was 66. 2% （43/65）, whereas COX-2 staining was weakly positive in 21 cases of 45 cases of paired breast tissues, which was significantly different from that in breast cancer （P〈0.01）. COX-2 expression was not associated with patient＇s age, tumor size, histological grading, TNM stage and lymph node metastasis, but it was correlated with the expression of VEGF, MMP-9, TIMP-1 and with MVD in invasive carcinomas. Conclusions The expression of COX-2 might promote the invasive potential of breast cancer through angiogenesis and metabolism of extracellular matrix.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2005年第4期442-445,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
乳腺肿瘤
环氧化酶
基质金属蛋白酶
组织金属蛋白酶抑制因子
微血管密度
血管内皮生长因子
免疫组织化学
breast neoplasms
cyclooxygenase-2
vascular endothelial growth factor
microvessel density
matrix metalloproteinase-9
tissue inhibitor of matrix metalloproteinase-1
immunohistochemistry
