摘要
合成了四种2,6-N,N-二[二(2-苯并咪唑甲基)]氨甲基-4-甲基苯酚(Hbbimp)的金属(Zn2+、Mn2+、Eu3+、Nd3+)配合物,并用红外、此外、摩尔电导、元互分析表征了它们的结构。用凝胶电泳实验研究了它们与PBR322DNA的作用,发现在37℃时四种金属配合物与DNA的作用效果均不明显;在50℃,pH8.0时,仅两种稀土(Eu3+和Nd3+)配合物可将大部分超螺旋DNA(CCC带)转化为缺刻产物(OC带);在37℃,[H2O2]<1×10-3mol/L,[稀土配合物]:[H2O2]=1∶20时,配合物在较低浓度时即可将CCC带全部转化为OC带.
The metal （Zn^2＋ , Mn^2＋ , Eu^3＋ , Nd^3＋ ） complexes of Hbbimp （ 2,6-N, N-bis [bis（2-benzimidazolylmethy） ] aminomethyl-4-methylphenoxo） were synthesized, and their structures were characterized with IR, UV-Vis,molar electorlytic conductivity and elemental analysis. Their research for cleaving DNA indicates the metal complexes can not cut DNA at 37℃ and only two rare-earth（ Eu^3＋ and Nd^3＋ ）complexes are capable of promoting cleavage of pBr322 DNA at 50℃ and translating most of the supercoiled DNA into nicked DNA. At the same time for the system of the rare earth complexes and H2O2, the supercoiled DNA can be absolutely translated into the nicked DNA at 37℃, [H2O2] 〈1×10^3mol/L, [complex]：[H2O2]=1： 20.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2005年第4期448-451,共4页
Chemical Research and Application
基金
国家自然科学基金资助项目(批准号:20171031)
