摘要
序列特异性ＤＮＡ断裂蛋白质是在序列特异性ＤＮＡ结合蛋白质及小分子ＤＮＡ断裂试剂基础上设计合成的。其基本原理是在含有ＤＮＡ结合域的蛋白质上引入一个金属螯合剂并螯合一个适当的金属离子，其中序列特异性ＤＮＡ结合蛋白质具有与ＤＮＡ特定序列结合的能力，从而可以起导向物的作用；而引入的金属螯合剂与金属离子复合物具有断裂ＤＮＡ的功能，两者协同作用可以达到序列特异性断裂ＤＮＡ的目的。
ased on the sequence-specific DNA-binding protein and DNA cleaving agents,complex of a chelator and an appropriate metal ion, sequence-specific DNA cleaving proteins were designed and synthesized. The DNA cleaving agents were directed to DNA by attachment to a DNA-binding protein, and the resulting protein may be used as a sequence-specific DNA cleaving protein.
出处
《化学进展》
SCIE
CAS
CSCD
1994年第1期14-25,共12页
Progress in Chemistry
基金
国家"攀登"项目
关键词
序列特异性
DNA
蛋白
断裂试剂
sequence-specific
DNA-binding protein
DNA cleaving agent
