摘要
目的:探讨成熟少突胶质细胞在细胞膜上是否有Nogo-A的表达及其比例,并收集该类活细胞。方法:不使用破膜剂,间接荧光标记细胞膜表达Nogo-A的成熟少突胶质细胞,采用流式细胞术分选被染色的阳性细胞。结果:经流式细胞术获得了细胞膜表达Nogo-A的成熟少突胶质细胞,比例为3.36%。结论:使用流式细胞技术,发现部分成熟少突胶质细胞在细胞膜表面有Nogo-A表达,其比例为3.36%。
Objective ： To explore the expression of Nogo-A in membrane of mature oligodendrocyte, and obtain these living cells. Methods：Mature oligodendrocytes were stained by indirect immuno-fluorescent technique. Stained unpermeable oligodendrocytes were sorted by flow cytometry. Results： The percentage of mature oligodendrocytes with Nogo-A expression in membrane was 3.36%. Conclusion ： No-go-A could be expressed in membrane of mature oligodendrocytes, and the ratio of positive cells was about 3.36%.
出处
《医学研究生学报》
CAS
2005年第8期690-691,695,共3页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30471775)
