摘要
用图像分析系统和通道阻断法研究了原代人胎儿鼻咽上皮细胞的调节性容积回缩(regulatoryvolumedecrease,RVD)能力及其机制。结果发现,低渗刺激可诱发鼻咽上皮细胞产生RVD,在160-240mOsmol/L范围内,RVD强弱与渗透压呈“S”形负相关(r=-0.99,P<0.05),与细胞肿胀程度呈“S”形正相关(=0.99,P<0.05)。Cl^-通道阻断剂tamoxifen(20μmol/L),ATP(10mmol/L)或NPPB(100μmol/L)对RVD阻抑率分别为100%(P<0.01),76.3%(P<0.01)和62.7%(P<0.01)。本研究表明,鼻咽上皮细胞受到低渗刺激时可产生RVD,Cl^-通道开放是其RVD的关键机制。
To investigate regulatory volume decrease （RVD） and its mechanism in primary-culturing fetal human nasopharyngeal epithelial cells, living cell imaging technique was employed to detect the volume changes following exposure to hypotonic solution, and blockage of Cl^- channels was used to clarify the role of Cl^- channels in RVD. The results showed that extracellular hypotonic treatment swelled the cells and induced RVD. 47% hypotonic solution （160mOsmol/L） swelled the cell by 144.7% and induced 38.7% recovery of cell volume within 20 min. RVD was correlated negatively to the extracellular osmolarity （r=-0.99, P〈0.05） and positively to the swelling volume（r=0.99, P〈0.05） in “S” shape, respectively. Chloride channel blockers, tamoxfen （20μmol/L）, ATP （10mmol/L） and NPPB （100μmol/L）, inhibited RVD by 100%, 76.3% and 62.7% （P〈0.01）, respectively. The results indicated that primary-culturing fetal human nasopharyngeal epithelial cells are capable of RVD. Cl^- efflux through Cl^- channels is the key mechanism of RVD.
出处
《实验生物学报》
CSCD
北大核心
2005年第4期353-358,共6页
Acta Biologiae Experimentalis Sinica
基金
英国威尔康姆基金(056909/299/Z)中国教育部基金(GJ9901)广东省卫生厅基金(A2001474)~~
