摘要
目的:检测多聚阳离子复合物(Polycation)对腺病毒载体转染血液肿瘤细胞效率的影响。方法:重组LacZ腺病毒(Ad-LacZ)联合Polycation分别转染HL-60、K562、Jurkat及Raji细胞,观察阳性细胞百分率。结果:Ad-LacZ单独转染肿瘤细胞时转染率较低。联合Polycation共同转染可明显提高腺病毒载体的转染率。结论:多聚阳离子复合物可做为提高Ad-LacZ转染HL-60、K562、Jurkat及Raji细胞的辅助工具。
Objective：To observe the influence of Polycafion on the transfecfion efficacy of recombinant adenovirus. Methods： Recombinant adenovirus coding Lac -Z gene adjuncted with Polycafion reagents were used to infect the hematologic malignant cell lines： HL- 60, Jurkat, Raji and K562. The transfection efficacy was determined by the X - gal staining. Results： Ad - LacZ showed a low transfection efficacy alone, the transfection efficacy could be enhanced by Polycation markedly. Conclusion： Polycafion can enhance the transfection efficacy of recombinant adenovirus in cell lines of hematological malignancies.
出处
《西北国防医学杂志》
CAS
2005年第4期250-252,F0002,共4页
Medical Journal of National Defending Forces in Northwest China
基金
国家"863"计划资助项目(Z20-01-02)
