摘要
目的:克隆化HCV非结构蛋白2(NS2)蛋白反式调节靶基因(NS2TP). 方法:依据我们构建的HCV NS2反式调节基因差异表达的cDNA消减文库筛选结果,利用分子生物学与生物信息学技术获得新基因NS2TP的编码序列,设计特异性引物,并对其进行克隆化研究. 结果:NS2TP基因编码区为456核苷酸(nt),编码产物为151氨基酸残基(aa),经核苷酸序列数据库(GenBank)和蛋白质一级结构序列数据库(SwissProt)同源序列的搜寻,与已知基因序列和蛋白序列之间没有显著同源性,属于未知功能新基因. 结论:发现了HCV
AIM： To clone and identify the new gene NS2TP transregulated by the non-structural protein 2 of hepatitis C virus. METHODS： Based on the subtractive cDNA library of genes transregulated by NS2 protein of hepatitis C virus, the coding sequence of the new gene was obtained by bioinformatics methods. Polymerase chain reaction （PCR） was conducted to amplify NS2TP gene. RESULTS： The coding sequence of new gene was cloned and identified successfully. The coding region of NS2TP gene had a length of 456 nucleotides and the coding product pocessed 151 amino acid residues. After searching in GenBank and SwissProt, the new gene had no significant homology with the genes we have known, and its functions remained unknown. CONCLUSION： A new target gene, transregulated by HCV NS2 protein, is recognized.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第14期1700-1704,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金攻关项目
No.C03011402
No.C30070689军队"九
五"科技攻关项目
No.98D063军队回国留学人员启动基金项目
No.98H038军队"十
五"科技攻关青年基金项目
No.01Q138军队"十
五"科技攻关项目
No.01MB135~~
