摘要
目的观察3周模拟失重对大鼠股骨生物力学特性及碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、胫骨Ⅰ型胶原含量的影响。方法青年雄性SD大鼠14只按体重配对后随机等分为对照组(CON)和模拟失重组(TS),采用尾部悬吊方法模拟失重,3周实验结束后处死大鼠,取新鲜股骨行三点弯曲法检测生物力学性状,取胫骨特殊化学染色法显示ALP,ACP,免疫组化法检测Ⅰ型胶原变化情况。结果与对照组相比,模拟失重组大鼠股骨弹性载荷、最大载荷均显著下降(P<0.01),最大挠度显著增大(P<0.05)。ALP染色显示TS组着色较对照组明显浅淡。ACP染色结果显示TS组较对照组阳性细胞明显增多。Ⅰ型胶原免疫组化染色显示TS组Ⅰ型胶原含量增加,排列不规则。结论3周模拟失重使大鼠后肢骨生长受抑,引起大鼠股骨生物力学性能的全面下降,ALP胶原代谢紊乱。
Objective To investigate effects of 3 weeks simulated weightlessness on biomechanical parameters, alkaline phosphatase （ALP）, acid phosphatase （ACP） and collagen Ⅰ of hind-limb bones in tail-suspended rats. Method Fourteen male SD rats were divided equally into control group （CON） and experimental group; tail-suspended（TS） was used to simulate weightlessness, After 3 weeks tail-suspension, biomechanical parameters of femur were measured; ALP,ACP and collagen Ⅰ of tibia were observed. Result Elastic load, maximum load, and bending rigidity coefficient decreased significantly（P 〈0. O1 ） ,while the maximum deformation and bending toughness coefficient increased markedly（P 〈0. O1 or P 〈0.05）. Morphological results showed that ALP declines significantly in TS group, ACP and type Ⅰ collagen increased significantly in TS group. Conclusion After tail-suspension for 3 weeks, growth of rat＇s weight bearing bones are suppressed, biomechanical capability declines, and collagen Ⅰ metabolism becomes disordered.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2005年第4期255-258,共4页
Space Medicine & Medical Engineering
基金
全军医药卫生科研基金资助(98Z083)
