摘要
目的:研究过量表达ErbB2对于MCF-7细胞生长和侵袭的作用。方法:构建带有ErbB2逆转录病毒,感染MCF-7细胞,检测ErbB2的表达。用转染后的细胞开展体外生长和侵袭实验。结果:转染后ErbB2在MCF-7细胞中过量表达。体外生长和侵袭实验显示,过量表达ErbB2的细胞增殖快、侵袭性强。结论:过量表达ErbB2促进MCF-7细胞的生长和侵袭;阻断ErbB2信号可能为高表达ErbB2的肿瘤治疗带来新的策略。
AIM： To investigate the effects of ErbB2 overexpression on growth and invasiveness in cultured MCF - 7 cell line. METHODS： Retrovirus containing ErbB2 gene was transfected into MCF - 7 cells and ErbB2 expression was detected by Western blotting. Proliferation and invasive assays were carried out. Cells overexpressed ErbB2 and its control, AP2, were used in the experiment. RESULTS： ErbB2 was overexpressed in MCF - 7 cells after tiansfection. In vitro, cells overexpressed ErbB2 showed highly proliferated and highly invasive characteristics compared to the control cells. CONCLUSION： Overexpression of ErbB2 promotes cell proliferation and enhances invasiveness in MCF - 7 cells. Inhibition of signaling induced by ErbB2 might be a novel strategy for the therapeutics of cancer with ErbB2 overexpression.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第8期1566-1568,共3页
Chinese Journal of Pathophysiology
基金
广东省科技推广基金资助(No.2004B20501001)
