摘要
目的探讨血清烯醇化酶，尤其是神经元特异性烯醇化酶（ｎｅｕｒｏｎ－ｓｐｅｃｉｆｉｃｅｎｏｌａｓｅ，ＮＳＥ）含量测定对视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，Ｒｂ）诊断和病情监测的价值。方法应用醋酸纤维薄膜快速电泳＋荧光分光光度法，对３５例经病理检查确诊的Ｒｂ患者，分别于眼球摘除时，术后３、６、１２和≥１８个月后及３１例对照组进行血清烯醇化酶总量和ＮＳＥ含量定量检测，观察比较血清烯醇化酶总量和ＮＳＥ含量的变化。结果Ｒｂ患者血清烯醇化酶总量为２０７．０±６５．９Ｕ／Ｌ，ＮＳＥ含量为９８．４±４０．３Ｕ／Ｌ，与对照组血清测定值（分别为１４０．７±４６．０Ｕ／Ｌ和４１．０±１０．９Ｕ／Ｌ）比较明显升高，两组间差异有显著性（Ｐ＜０．００１）。８２．６％Ｒｂ患者血清ＮＳＥ含量明显高于对照组上限水平。Ｒｂ患儿术后６、１２和≥１８个月血清烯醇化酶总量和ＮＳＥ含量逐渐下降（ＮＳＥ含量下降较缓慢），与治疗前的测定值相比差异有显著性；至≥１８个月后血清ＮＳＥ含量和烯醇化酶总量降至对照组测定值水平。结论血清烯醇化酶尤其ＮＳＥ含量测定对Ｒｂ患者有一定的辅助诊断价值。
Objective To investigate the value of determining quantity of neuron-specific enolase （NSE） in the diagnosis and supervision in patients with retinoblastom a （Rb）. Method We used rapid electrophoretic and the fluorometer method in the 35 Serum specimens from 35 patients with Rb during surgery, aftet enucleation , respectively, at 3, 6, 12,≥ 18 months and 31 serum specimens from 31 control cases to detetmine the quantity of NSE and cnolase isoenzymes. Results There were 207± 65. 9 u/L of all isoenzymes and 98. 4± 40. 3 u/L of NSE from 35 patents with Rb. In comparison with the levels of control cases （ respectively, 140. 7± 46. 0 U/L of all isoenzymes, 41±10. 9 u/L of NSE）, both serum levels in patients with Rb increased obviously, the difference being signifrcant. At 6, 12, and≥ 18 months after surgery, the determ inations of all isoenzymes and NSE from 35 patients with Rb decreased step by step. the difference being significant in comparison with the values before the surgery. After surgely ≥ 18 months, the levels of NSE and all enolase decreased tothat of NSE and all enolase from control cases. Conclusion The determination of enolase level, especially NSE of serum ,is valuable for the diaguasis of patients with Rb.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1998年第2期117-120,共4页
Chinese Journal of Ophthalmology
