摘要
目的探讨慢性应激对大鼠海马和前脑皮层神经颗粒素(NG)含量的影响。方法将雄性SD大鼠随机分为应激组(予10℃冷水游泳应激,共2周)、装置对照组和正常对照组,每组10只。于应激前后测量三组大鼠的体质量变化情况,并记录每天应激过程中应激组和装置对照组的排便量以考察应激的强度,应激后以Westernblotting方法测定海马和前脑皮层中的NG含量(以NG含量与βActin含量的比值表示)。结果(1)应激前后,三组大鼠的体质量比较,差异均无统计学意义(P>0.05),但应激组的体质量增长相对缓慢;(2)应激第4—14天应激组的排便量多于装置对照组(P<0.01);(3)应激组和装置对照组海马的NG含量[分别为(0.66±0.13)和(0.94±0.26)]低于正常对照组[(1.93±0.53)],差异有统计学意义(P<0.01);(4)应激组前脑皮层的NG含量[(0.45±0.00)]低于装置对照组和正常对照组[分别为(2.76±1.74)和(2.87±1.63)],差异有统计学意义(P<0.01);(5)应激组海马NG含量高于前脑皮层(P<0.01);装置对照组海马NG含量低于前脑皮层(P<0.01);正常对照组海马与前脑皮层NG含量的差异无统计学意义。结论慢性应激导致海马和前脑皮层NG含量显著下降;对于时程长、程度严重、适应不良的冷水游泳应激,前脑皮层受损比海马更为严重;而对于时程长、程度微弱的装置应激,海马则比前脑皮层更为敏感。
Objective To investigate the effects of chronic cold water swimming stress on protein levels of neurogranin in rat hippocampus and forebrain, and to understand the central mechanisms of stress from the molecular level. Methods Thirty SD rats were randomly divided into handled-controls, apparatuscontrols and stressed groups, with ten in each group. Forced 10℃ cold water swimming was used as a stressor. The defecation numbers of both stressed rats and apparatus controls were recorded during 5-minute trial on each day. Furthermore, the weight of rats was monitored during the periods of stress as an indexre flecting degree of stress. The neurogranin level in the hippocampus and forebrain after stress was measured with the Western blotting. Results （ 1 ） Although there was no significant difference in final body weight among the three groups, less weight gain was in stressed rats than in controls. （2） In comparison with the apparatus controls, the stressed group had significantly more numbers of defecation during a 5-minute trial on the 4th to 14th experiment day （ P 〈0. 01 ）, and no significant differences were found between two groups ont he experiment day 1 to 3 （ P 〉 0. 05 ）. （ 3 ） the hippocampal protein levels of neurogranin in stressed group （0. 66±0. 13） and apparatus-controls （0. 94±0. 26） were significantly lower than that in handled-controls（1.93±0. 53 ） （P 〈 0. 01 ）. （4） The forebrain protein levels of neurogranin in stressed group （0. 45±0. 00） were significantly lower than that in apparatus-controls （2.76±1.74） and handled-controls （2. 87±1.63 ） （P 〈0. 01 ）. （5） The protein levels of neurogranin in hippocampus were significantly higher than that in forebrain （ P 〈 0. 01 ） in stressed group. However, the protein levels of neurogranin in apparatus-controls was significantly higher in forebrain than in hippocampus （ P 〈 O. O1 ）. There was no significant difference between forebrain and hippocampal neurogranin levels in h
出处
《中华精神科杂志》
CAS
CSCD
北大核心
2005年第3期174-177,共4页
Chinese Journal of Psychiatry
基金
国家自然科学基金资助项目(30370482)
中国科学院创新工程资助项目(KSCX2203)
