摘要
用简易的髓鞘碱性蛋白（ＭＢＰ）及其抗体（Ａｎｔｉ－ＭＢＰ）酶联免疫吸附定量测定（ＥＬＩＳＡ）法对６组共３３７例神经系统疾病患者进行检测，其中脊髓压迫症（ＣＭＰ）３６例，多发性硬化（ＭＳ）３３例，脑血管疾病（ＣＶＤ）３４例，中枢神经系统感染性疾病（ＩＤ）３１例，癫痫（ＥＰ）１６１例和其他神经系统疾病（ＯＮＤ）４２例。结果显示：ＣＭＰ、ＭＳ．ＣＶＤ、ＩＤ和ＥＰ５组血清ＭＢＰ均值明显高于ＯＮＤ组（Ｐ＜０．０１），其中又以急性外伤所致脊柱骨折伴截瘫为主（３３例）的ＣＭＰ患者血清ＭＢＰ值最高，与ＭＳ组比较Ｐ＜０．０５，与ＣＶＤ、ＩＤ和ＥＰ组比较Ｐ＜０．０１。ＣＶＤ患者ＣＳＦＭＢＰ均值也明显高于ＯＮＤ组（Ｐ＜０．０５）。本法检测６组血清和ＣＳＦ的Ａｎｔｉ－ＭＢＰ含量差异不显著（Ｐ＞０．０５）。
Simplified enzyme-linke immunoad-sorbent assays(ELISA)for myelin basic protein (MBP) and antibodies to myelin basic protein(Anti-MBP)have been used to test 337 patients with dis-eases of the nervous system including 36 compressive diseases (CMP),33 multiple sclerosis (MS),34 cere-brovascular diseases(CVD),31 inflammatory diseases of central nervous system (ID),161 epilepsy(EP)and 42 other nervous diseases(OND ).Comparison of results among various groups indicates that serum mean MBP values of CMP,MS.CVD,ID and EP groups are significantly higher than those of ONDgroup and normal control(P<0. 01).The serummean MBP value of 33 acute trauma patients withspine fracture and paraplegia, the majority of CMPgroup, is the highest compared with MS group(P<0.05),CVD,ID and EP groups(P<0.01).CSFmean MBP value of 15 CVD patients is markedlygreater than that of OND group (P<0. 05). No sta-tistlcally significant differences are found in serurnMBP values between OND group and normal control,and in serum and CSF Anti-MBP values among sixgroups by using our method.
出处
《华西医科大学学报》
CSCD
1995年第2期131-134,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
四川省科委科研基金
关键词
神经系统疾病
髓鞘碱性蛋白
ELISA
血清
脑脊液
Diseases of the nervous systern Myelin basic protein Antibodies to myelin basic protein Enzyme-lindec immunoadsorbent assay
