摘要
本文应用单克隆抗体OKT系列检测了33名支气管哮喘儿童外周血的T淋巴细胞(OKT3^+细胞)、T辅助细胞(OKT4^+细胞)和T抑制/杀伤细胞(OKT8^+细胞)。采用间接免疫荧光法和流式细胞仪检测了口服雷公藤多甙前后T细胞亚群数量的变化:服药后OKT4^+细胞明显减少而OKT8^+细胞明显增高,T4/T8比值明显降低(P<0.05)。上述结果表明雷公藤多甙对哮喘儿童的T淋巴细胞亚群的分布确有影响,也进一步证明了雷公藤及其制剂的免疫抑制作用。
In this study, 33 cases of asthmatic children was included. All subjects had positive skin prick test to 18 antigens and were treated with GTW for one week. The dosage of GTW was 1-1.5mg/kg/d. We examined the proportion of peripheral blood T cells by using the OKT monoclonal anti-human T lymphocyte antibodies, that is OKT3 ( most T cells ) ,OKT4 ( helper ) and OKT8 ( suppressor/cytoto-xic) . Each sample was examined by indirect immunofluorescence method with flow cytometer.
The T subsets, before and after the treatment with GTW, were detected and compared with each other. We found that after GTW treatment, there were a significant increase in the number of the OKT8+ cells and a significant decrease in number of the OKT4+ cells, that caused a remarkable fall of T4/T8 ratio(P<0.05).
We concluded that the GTW do have the important effects on the proportion of T cell subsets,that may be one of the important reasons for the potent immuno-suppressive actions of GTW.
出处
《第一军医大学学报》
CSCD
1989年第2期102-105,共4页
Journal of First Military Medical University
关键词
雷公藤多甙
T细胞
哮喘
儿童
glycosides of tripterygium wifordii
T lymphocyte subpopulation
asthmatics
