摘要
采用限制性内切酶消化、琼脂糖凝胶电泳及分子杂交技术对８株中国伊氏锥虫动基体ＤＮＡ（ｋＤＮＡ）微环进行了比较研究。结果显示，我国伊氏锥虫株之间的ｋＤＮＡ微环序列具有较高的同源性，仅限制酶 ＡｌｕＩ，ＨｉｎｆＩ及 ＭｂｏＩ的酶解结果显示少数虫株的 ｋＤＮＡ微环存在异源序列。这种异源性可以作为伊氏锥虫种内分类的遗传学标志。
Restriction endonucleases digestion of kinetoplast DNA minicircle, combined with agarose gel electrophoresis and molecular hybridization to characterize 8 Trypanosoma evansi strains in China was performed. The results presented in restriction enzymes digestion showed that all minicircles of 8 T. evansi strains had identical electrophoretic patterns of restriction fragment with Dde I, Taq I and Sin I. However, the polymorphisms of minor restriction fragment length were observed with Alu I, Hinf I and Mbo I, the minicircles of 6 strains (YNB, ZJB, JSB2, HNB, HBM, GDH) were cleaved once by Hinf I, twice by Mbo I and none by Alu I, and had identical restriction fragment patterns; the minicircles of 2 other strains (XJCA, AHB) were cleaved once by Mbo I, Alu I and twice by Hinf I, When the probe PTK1 was used to hybridize to Hinf I digested fragments, 2 minicircle classes were found with AHB strain. The 8 T. evansi strains could be divided into 2 minicircle types (type A-YNB, ZJB, JSB2, HNB, HBM, GDH and type B-XJCA, AHB) and the type B could be further diveded into type B1 (XJCA)and type B2 (AHB)based on the results in this inves- tigation. The evidence provided by present investigation showed that Chinese T. evansi strains shared kDNA minicircle Sequence homology with each other, but also showed a minor minicircle sequence heterogeneity. It is suggested that low genetic variation occur in Chinese T. evansi, and all strains form a homogeneous species group. However, the above-mentioned minicrcle sequence heterogeneity might be used as a tool for intraspecies classification of T. evansi
出处
《动物学报》
SCIE
CAS
CSCD
1995年第1期88-95,共8页
ACTA ZOOLOGICA SINICA
基金
国家自然科学青年基金资助项目
批准编号39000079
关键词
伊氏锥虫
动基体DNA
限制性内切酶
Trypanosoma evansi, Kinetoplast DNA, Intra-species classification, Restric- tion endonuclease, Molecular hybridization.
