摘要
旨在构建猪AQP11基因高效表达载体,观察该载体转染Hela细胞的转染效果。本研究通过PCR方法从猪肠道黏膜组织中克隆AQP11基因,构建了微环表达载体minicircle-AQP11,通过瞬时转染Hela细胞观察绿色荧光蛋白表达。结合测序结果表明,本研究成功地克隆了AQP11基因的全序列,包含了完整的CDS区,构建了AQP11基因的微环表达载体,在Hela细胞中观察到了持续1周的绿色荧光蛋白表达。这为进一步阐明AQP11合成的生化过程及功能调控提供新思路。
This study aimed to construct minicircle expression vector of AQP11 and observe the effect of transfection in Hela cells.AQP11 gene was amplified from the porcine intestinal mucosa and minicircle expression vector minicircle-AQP11 was constructed.The recombinant plasmid minicircle-AQP11 was transfected into Hela cells to investigate the expression of GFP by green fluorescent microscope.The sequencing results showed that the whole sequence of AQP11 gene containing a whole CDS was amplified successfully.Minicircle expression vector minicircle-AQPl1was constructed successfully.GFP was successfully expressed in Hela cells by the detection of bright green fluorescence for one week.Which provide new ideas for further elucidating the biochemical process and functional regulation of AQP11.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第1期9-13,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
农业部"引进国际先进农业科学技术"(948)重点项目(2011-G35)
