摘要
以人骨肉瘤细胞系（ＨＯＳ－８６０３）为模型，首先研究了腺苷酸环化酶激活剂Ｆｏｒｓｋｏｌｉｎ和地塞米松（Ｄｅｘ）对该细胞系增殖分化过程的影响。结果发现，Ｆｏｒｓｋｏｌｉｎ本身无明显作用，但可加强Ｄｅｘ对ＨＯＳ－８６０３细胞克隆增殖的抑制作用，并促进Ｄｅｘ对碱性磷酸酶活性的诱导。为进一步阐明Ｆｏｒｓｋｏｌｉｎ加强Ｄｅｘ对ＨＯＳ－８６０３细胞增殖分化调节作用的机制，进一步研究了其对糖皮质激素受体（ＧＲ）的调节作用。证明Ｆｏｒｓｋｏｌｉｎ可使ＧＲ最大结合容量明显升高，而且具有时间依赖性特点，但亲和力无明显改变。结果表明：Ｆｏｒｓｋｏｌｉｎ加强糖皮质激素对ＨＯＳ－８６０３增殖分化调节作用是通过增量调节ＧＲ实现的。
The effect of forskolin,an activator of adenylate cyclase, on glucocorticoid-induced modulation of proliferation and differentiation of a human osteasercoma cell line (HOS-8603) was initially studied.It was found that forskolin could significantly augment the inhibitory effect of glucocorticoids on HOS-8603 cell clonal proliferation and enhance the induction of alkaline phosphatase (AKP) activity by glucocorticoids even thouth forskolin alone had no effect. To clarify the mechanism responsible for the augmenting effect of forskolin on glucocorticoid-induced cellular effect on HOS-8603 cells,the effect of forskolin on glucocorticoid receptor(GR) level was further evaluated.It was shown that forskolin could significantly up-regulate GR level in a timedependent manner with no concomitant change in GR binding affinity for glucocorticoids. These results suggest that the augmentation of glucocorticoid effect on HOS-8603 cell proliferation and differentiation by forskolin is achieved by up-regulation of GR.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1995年第1期17-20,共4页
Academic Journal of Second Military Medical University
