摘要
目的研究顺铂诱导HOS-8603细胞凋亡与线粒体跨膜电位(ΔΨm)关系。方法MTT法测定顺铂对HOS-8603细胞的抑制率;将HOS-8603细胞分别用顺铂、顺铂+1.0ug/ml环孢菌素A(CsA)处理,用透射电镜观察HOS-8603细胞形态学变化;用流式细胞术分别测定亚G1期细胞百分率及PI和Rh123双染色后细胞的ΔΨm。结果MTT还原试验表明,作用24小时后,1ug/ml和50ug/ml顺铂的抑制率分别为58.56%,90.69%;HOS-8603细胞经顺铂作用后,透射电镜下观察到典型的凋亡形态特征;随着顺铂作用时间增加,HOS-8603细胞凋亡数增加和ΔΨm降低(P<0.01),两者呈直线相关,CsA能部分抑制这些效应。结论顺铂诱导HOS-8603细胞凋亡的另一途径是通过使线粒体膜通透性转运孔开放,ΔΨm降低来实现的。
Objective To explore the relationship between Hos-8603 cell apoptosis induced by Cisplatin and the cell mitochondrial transmembrane potential(ΔΨm) . Methods Cisplatin-mediated cytotoxicity by MTT assay in HOS-8603 osteosarcoma cell line;Hos-8603 cells were treated with Cisplatin and Cisplatin plus 1. 0μg/ ml CsA respectively. Morphological changes were observed under light microscope and transmission electron microscope. The percentages of subG1 cells andΔΨm of cells doublely stained with PI and Rh123 were assayed by flow cytometry. Results HOS-8603 cells were treated for 24 hours with different concentration of Cisplatin and the inhibitive rates of Cisplatin were evaluated with MTT assay ,the inhibitive rates of 500ug/ml,1ug/ml,5ug/ml,10ug/ml and 50ug/ml Cisplatin inducing HOS-8603 osteosarcoma cell line were 33.6%, 58.56%,72.33%, 80.18% and more than 90% respectively. After treatment with Cisplatin, Hos-8603 cells appeared the classical apoptotic morphology. The percentages of subG1 cells were increased , while theΔΨm reduced ( P< 0. 01) and there was a linear correlation between them. The increment of subG1 cell percentages and decrement of ΔΨm induced by Cisplatin were partly inhibited by CsA (P< 0. 01) . Conclusion Cisplatin can partly induce Hos-8603 cell apoptosis through opening the mitochondrial permeability transition pore and reducingΔΨm , and this effect could be partly inhibited by CsA.
出处
《现代肿瘤医学》
CAS
2005年第3期301-303,共3页
Journal of Modern Oncology
基金
湖北省自然科学研究基金资助项目(基金编号:2003ABA163)
