摘要
目的 研究塞来昔布(celecoxib)对人骨肉瘤细胞株HOS-8603生长和凋亡的影响,并探讨其作用机制。方法 采用四唑盐(MTT)比色法检测细胞增殖,以确定塞来昔布对人骨肉瘤细胞株HOS-8603的有效剂量;应用高效液相色谱(HPLC)检测前列腺素E2(PGE2 )含量;光镜形态学观察,流式细胞仪测定细胞周期。结果 塞来昔布抑制人骨肉瘤细胞株HOS-8603生长和诱导凋亡(当celecoxib的浓度为80umol/L时抑制率达到46. 75 ±7. 697% );这种抗增殖作用可能与celecoxib对COX-2的抑制作用有关;且不能被PGE2所拮抗。塞来昔布能够诱导HOS-8603细胞凋亡,当其浓度为80umol/L时,凋亡率增加了44. 7% (46. 0%比1. 3% );但是塞来昔布的这种抑制HOS-8603细胞生长和诱导凋亡作用不能被PGE2 所拮抗。结论 塞来昔布对于人类骨肉瘤可能是一种有效的化学治疗和化学预防药物,塞来昔布并非通过前列腺素E2(PGE2 )途径抑制人骨肉瘤细胞株HOS-8603生长和诱导凋亡。
Obiective To evaluate the effects and mechanisms of ac tion of celecoxib in inhibiting proliferarion and inducing apoptosis on human os teosarcoma cells.Methods The proliferarion of human osteo sarcoma cell line-HOS-8603 was measured by MTT assay.The lever of prostaglandin E2 secreted by osteosarcoma cell was detected by High Performance Liquid Chromat ogram assay.Apoptosis was measured using FACS.Results The inhibitory rate of celecoxib on HOS-8603 cell was 46.75 ± 7.697%,if HOS-8603 cell has been treated with 80umol/L celecoxib for 48 hours. By FACS, it was fou nd that the rate of apoptosis cells were increased up to 46.0%(control group was 1.3%). The result of Western Blot assay revealed that apoptosis rates of differ ent concentrations of celecoxib accorded with the content of Cyclooxygenase-2. But these effects of celecoxib on HOS-8603 cells can't be counteracted by prosta glandin E 2:When we added 1.5mg/L prostaglandin E 2 to cells that were treated with 80umol/L celecoxib,the inhibitory rate and apoptosis rate were 46.30 ± 6. 812% , 45.8%(control group was 1.3%). Conclusion Celecoxi b played an inhibitory effect and induced apoptosis on HOS-8603 cells. But these effects of celecoxib can't be counteracted by prostaglandin E2.So their proba ble mechanism had nothing to do with prostaglandin E 2.
出处
《现代肿瘤医学》
CAS
2005年第2期174-177,共4页
Journal of Modern Oncology
基金
湖北省科技攻关计划基金资助 (项目编号: 2004AA304B08)
