摘要
从滇产苦绳(Dregea sinensis var.corrrugata)的根茎中分得两个新的C_(21)-甾体甙元,命名为苦绳甙元Ⅰ及Ⅱ(drevogenin Ⅰ,Ⅱ),经光谱分析和化学反应证明,甙元Ⅰ的结构为C/D顺式5α-H,3β,8β,14β,17β四羟基-12β-O-异戊酰基-20-O-异戊酰基娠烷(C/D cis 5α-H,3β,8β,14β,17βtetrahydroxy一12β-O-isovaleryl-20-O-isovaleryl-pregnane)。甙元Ⅱ的结构为C/D顺式5α-H,3β-14β,17β三羟基-12-β-O-乙酰基-20-O-苯甲酰基娠烷(C/Dcis 5α-H,3β,14β,17βtrihydroxy-12β-O-acetyl-20-0-benzoyl-pregnane)。
Drevogenin Ⅰ, Ⅱ, two new aglycones, were isolated from Dregea sinensis var. corrugata(Asclepiadaceae). The mass spectrum suggests the presence of two isovaleryl groups.Alkaline hydrolysis of compound Ⅰ with 5% methanolic potassium hydroxide yielded deacyldrevogenin Ⅰa and isovaleric acid.The acid was esterified with methanol--surfuric acid to afford methyl-isovalerate. By GLC comparison with authentic sample,relative retention time was the same,~1H-NMR shift (δppm) and coupling constant were the same, too. On the basis of spectroscopic analysis and chemical reactions, given above the structure of aglyeone Ⅰ was established as C/D cis 5α-H,3β,8β,14β,17β tetrahydroxy- 12β-O-isovaleryl-20-O-isovalerylpregnane. MS moleculer weight 552 and elementary analysis were compatible with C_(?)H_(?)O_(?).
The structure of aglycone Ⅱ was established as C/D cis 5α-H,3β,14β,17β trihydroxy-12β-O-acetyl-20-O-benzoyl-pregnane,The mass spectrum suggests the presence of benzoyl and acetylgroups. Alkaline hydrolysis of Ⅱ with 5% methanolic potassium hydroxide yielded tomentogenin Ⅱa and an acetic acid. Partial alkaline hydrolysis of Ⅱ with 8% K_2CO_3--MeOH--H_2O afforbed a 12-hydroxy-20-O-benzoyl-pregnane.
出处
《药学学报》
CAS
CSCD
北大核心
1989年第8期587-592,共6页
Acta Pharmaceutica Sinica
关键词
苦绳
甾体成分
苦绳甙元
Dregea sinensis var.corrugata
Drevogenin Ⅰ,Ⅱ
Asclepiadaceae
