摘要
为探讨丙型肝炎ＩｇＭ抗体的临床意义，建立了间接酶联免疫吸附法（ＥＬＩＳＡ），该方法重复性好、特异性强、操作简便。检测２２例急性丙型肝炎和５０例慢性丙型肝炎患者的血清，结果单独使用核心区抗原检测ＩｇＭ抗体的阳性率大于用核心区和非结构区３抗原共同检测。抗－丙型肝炎病毒（ＨＣＶ）ＩｇＭ在急性丙型肝炎患者中检出率为８６％，慢性丙型肝炎中检出率为６６％，但急性组中５０％患者ＩｇＭ出现（约于输血后７．０±３．７周即可在血清中测出）早于ＩｇＧ，而慢性组中无一例如此，两组间差异有非常显著意义（Ｐ＜０．００５）。急性组血清丙氨酸转氨酶显著升高患者中８０％可测出ＩｇＭ；慢性组中６７％可测出ＩｇＭ。ＨＣＶＩｇＭ对急性丙型肝炎的诊断是一个有价值的指标，同时与疾病的活动性有一定相关性。
e set up an ELISA method for the detection of IgM antibody to hepatitis C virus in order to search the clinical significance of HCV IgM. This method was simple and convenient with high specifity and sensitivity. IgM antibody was tested in 22 patients with acute posttransfusion hepatitis C and 50 pa-tients with chronic hepatitis C. The result showed that the positive rate of IgM to Core region was high-er than that of IgM to Core+NS3 region. Anti-HCV IgM was detected positively in 86% of acute cases and 66% of chronic cases,but there was 50% patients whose IgM appeared(can be detected in the serum in about 7.0±3.7 weeks after transfusion)earlier than IgG in the acute hepatitis group,whereas in the chronic group,there was no case whose IgM emerged earlier than IgG,so there was obvious dif-ference between these two groups.We could detect IgM in 80% patients with significant elevation of ALT in acute hepatitis C group. In the meanwhile,there was 67% patients with HCV IgM positive in chronic group of ALT elevation. It is demonstrated that HCV IgM is a valuable maker in the diagnosis of acute hepatitis C and also the presence of correlation with the activity of hepatitis C shown.
