摘要
用丙型肝炎病毒(HCV)结构区(C区)和非结构区(Ms3区)制备固相抗原,建立了检测HCV IgM抗体的间接ELISA,并用此法检测了101份丙型肝炎病人血清。检测结果表明,急性输血后丙型肝炎病人和HCV抗体阳性伴ALT升高者,其抗C区IgM阳性者所占比例明显大于抗Ns3区。单独用C区抗原检测IgM抗体,其阳性率大于用C区和Ns3区抗原共同检测的结果。前者Cut off指数(COI)>2者占阳性例数的71.5％,后者仅占26％。因而显示了HCV结构区抗原在IgM检测中的重要意义以及抗C区IgM阳性有可能成为新近感染或活动性丙型肝炎的标志。
A ELISA for testing IgM antibody to hepatitis C was developed with solid phase HCV structure antigen (core region) and nonstructure antigen (Ns3 region). 101 hepatitis C patients sera were tested. The results showed that positive ratio of IgM to core region (core positive/positive) was higher than that of IgM to Ns3 region (Ns3 positive/positive) in acute post-transfusion hepatitis C patients and in those anti-HCV positive with ALT-elevated pateints. Anti-HCV IgM positive ratio tested by core region antigen was higher than that by core region+ Ns3 region. The ratio of COI>2 accounted for 71. 5% among those positive tested by core region antigen and 26% among those tested by core region+ Ns3 region antigen. It was demonstrated that HCV structure antign was important in examination of IgM antibody to hepatitis C,anti-HCc IgM might be a marker indicating recent HC infection and perhaps also a marker for active HCV infection.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1995年第4期254-257,共4页
Chinese Journal of Microbiology and Immunology
