摘要
用表达２型单纯疱疹病毒（ＨＳＶ－２）糖蛋白Ｄ的重组痘苗病毒（Ｒ－ｇＤ－Ｖ）免疫ＣＢＡ小鼠，通过间接免疫荧光（ＩＦ）（观察小鼠抗体滴度变化）和四甲基偶氮唑蓝法（ＭＴＴ）动态观察细胞毒性Ｔ细胞活性（ＣＴＬ），均见增高并检测了其产生的抗体对动物的保护作用。同时对Ｒ－ｇＤ－Ｖ和２型单纯疱疹病毒（ＨＳＶ－２）产生的二次ＣＴＬ活性进行了比较，结果表明，Ｒ－ｇＤ－Ｖ产生抗体３周达高峰（１：６４０），７周下降为１：３２０，抗体对病毒攻击动物有保护作用，其中和指数（Ｎ）为６３。Ｒ－ｇＤ－Ｖ在鼠体内产生初次ＣＴＬ８ｄ达高峰，１２ｄ后消失；二次ＣＴＬ持续８周之久，且ＣＴＬ活性明显高于初次。Ｒ－ｇＤ－Ｖ和ＨＳＶ－２相比较产生ＣＴＬ在统计学上有差异（Ｐ＜０．０５）。说明Ｒ－ｇＤ－Ｖ能引起体液免疫和细胞免疫，对动物有保护作用。
CBA mice were immunized with recombinant vaccinia virus expressing herpes simplex virus typeⅡGlycoprotein D (R-gD-V).With indirect fluorescence and Thiaxolyl blue tetraxoliu, bromide (MTT) method, we observed iccreased antibody titer, cytotoxic T lymphocyte activities and protective ability of antibody on mice , the secondary in vitro cytotoxic Tlymphocyte(CTL)activities of R-gD-V and herpes simplexvirus typeⅡ(HSV-2).The result showed that antibody titer of R-gD-V reached its peak (1:60)in the 3st week affter immunization.The antibody showed a protective ability on mice challenged with GSV-2 .The neutralixing antibody index was 63. Primary CTL reached its peak on the 8th day and disappeared on the 12th day. The seconclary CTL remained a leval higher than primary CTL for 8weeks. There was a statistically significant difference between the CTL induced by R-gD-V and HSV-2(P<0.05).Taken together R-gD-Vinduced cellualar and humaral immunities could protect the mice challenged with tacked by GSV-2.
出处
《中华实验和临床病毒学杂志》
CSCD
1995年第3期246-249,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
疱疹病毒
痘苗病毒
疫苗
免疫
病毒蛋白
糖蛋白
Recombinant vaccinia virus
MTT method
Cytotoxic T lymphocyte
Neutralized antibody index .
