摘要
应用ＢＡ－ＥＬＩＳＡ酶联免疫技术、免疫组化和油红Ｏ组织化学等方法观察了氧化ＬＤＬ对巨噬细胞清道夫受体活性的影响和前列腺素Ｅ＿２（ＰＧＥ＿２）等的保护作用。结果显示，氧化ＬＤＬ组的巨噬细胞含脂量、清道夫受体活性及免疫组化阳性颗粒三项指标均明显高于ＰＧＥ＿２和亚硒酸钠两用药组。表明ＰＧＥ＿２和亚硒酸钠均有明显抗氧化作用，而ＰＧＥ＿２作用还略优于抗氧化剂亚硒酸钠。
he influence of oxidized LDL(OX-LDL)on scavenger receptor of macrophages and the protective effects of PGE_2 and selenium was studied by means of BA-ELISA technique, immunohistochemical methods and oil red O histochemistry。The results indicated that the scavenger receptor activities of macrophages in two treated groups were much lower than that of OX-LDL group。Oil red O staining showed that the macrophages in OX-LDL group were full of fatty drops and became foam cells while lit-tle lipid could be found in the treated groups.Immunohistochemical study revealed that the entry of OX-LDL into the macrophages was by way of scavenger receptors. The results confirmed that both PGE_2and selenium had marked antioxidizing effects and that PGE_2 was slightly more effective than selenium.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1995年第3期165-167,共3页
Chinese Journal of Pathology
基金
国家自然科学基金
