摘要
目的:建立RP-HPLC法测定茯苓中去氢乙酰茯苓酸的含量。方法:采用Phenomenex LunaC_(18)柱(4.6mm×150mm,5μm),以乙腈:0.05%H_3PO_4水(64:36,V/V)为流动相,流速1.0ml/min,检测波长242nm,柱温25℃,用外标法定量,测定茯苓中去氢乙酰茯苓酸的含量。结果与结论:去氢乙酰茯苓酸的线性范围0.35～1.75μg,相关系数r=0.9991,回收率97.86%,RSD1.52%,该方法简便,快速,线性关系良好。
Objective: To determine the content of dehydropachymic acid in Poria cocos. Methods: The RP-HPLC methodwas used to determine the content of dehydropachymic acid in Poria cocos. The analysis was carried out on Phenomenex LunaC18 column(4.6mm×150mm,5μm). The mobile phase was CH3CN-0.05%H3PO4 (64:36, V/V), flow-rate 1.0ml/min, wavelength242nm and temperature 25℃. Results and conclusion: The method was simple and had a good linear relationship. The liner rangewas 0.35～1.75μg and gave a correlation (r) of 0.9991. The recovery was 97.86% and RSD 1.52%. The method was easy todetermine the content of dehydropachymic acid in Poria cocos.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第7期198-199,共2页
Food Science
