摘要
背景肝内阻力增加是门静脉高压的启动因素,内皮素在其中起重要作用;而高动力循环状态是门静脉高压的重要维持因素,其形成与一氧化氮(NO)的作用密不可分。但上述结论大多建立在肝硬化门静脉高压的基础上,而阻塞性黄疸早期尚未形成肝硬化时并发门静脉压力升高的机制尚不清楚。目的观察阻塞性黄疸大鼠门静脉压力的变化,探讨NO在阻塞性黄疸早期门静脉高压中的作用。方法将Sprague鄄Dawley大鼠随机分为假手术组和胆总管结扎组,分别于术后3、7、14天比较两组的游离门静脉压力(FPP)、血清和肝组织NO含量以及一氧化氮合酶(NOS)活性。结果大鼠胆总管结扎7天后,其FPP显著高于假手术组(P<0.05);而血清、肝组织NO含量和肝组织NOS活性仅在结扎第3天时显著高于假手术组(P<0.05),此后即与假手术组无显著差异。胆总管结扎组大鼠的FPP与血清和肝组织NO含量无相关性。结论阻塞性黄疸早期门静脉压力升高与NO无相关性。
Background: The increase of intrahepatic resistance is a triggering factor for portal hypertension, of which, endothel-in plays an important role in this process. The maintenance of portal hypertension in hyperkinetic circulatory state is closely related to nitric oxide (NO) formation. However, all the above conclusions are predominantly based on cirrhotic portal hypertension, and the mechanism of noncirrhotic portal hypertension in early stage of obstructive jaundice is still uncertain. Aims: To observe the changes of portal venous pressure in rats with obstructive jaundice, and to study the effect of NO on portal hypertension in early stage of obstructive jaundice. Methods: The Sprague-Dawley rats were divided randomly into sham operation group and bile duct ligation group. The free portal pressure (FPP), serum and hepatic NO and nitric oxide synthase (NOS) levels were compared between the two groups at the 3rd, 7th and 14th day postoperatively. Results: In bile duct ligation group, the FPP was significantly higher than that in the sham operation group at the 7th and 14th day postoperatively (P<0.05), but the serum and hepatic NO and hepatic NOS levels in bile duct ligation group were similar to those in sham operation group except the 3rd day postoperatively (P<0.05). No relationship was found between FPP and the serum and hepatic NO levels in bile duct ligated rats. Conclusions: The development of portal hypertension in early stage of obstructive jaundice is not correlated with NO.
出处
《胃肠病学》
2005年第3期168-170,共3页
Chinese Journal of Gastroenterology
