摘要
目的研究白细胞介素(IL)1β和肿瘤坏死因子α(TNFα)对酰基辅酶A胆固醇酰基转移酶(ACAT)mRNA水平的影响,探讨动脉粥样硬化形成的机制及其调节水平。方法在小鼠主动脉平滑肌细胞(VSMC)及肌源性泡沫细胞(FC)中分别加入IL1β和TNFα,孵育24h,用半定量RTPCR检测ACATmRNA表达。结果①无论有无细胞因子的干预,VSMC转变为FC后,ACATmRNA的表达均增加。②TNFα对VSMC和平滑肌源性FC中ACATmRNA的表达无影响。③IL1β能促使平滑肌源性FC中的ACATmRNA的表达。结论IL1β在转录水平上调ACATmRNA的表达,促使胆固醇的酯化和胆固醇的沉积,使VSMC向FC转化,参与动脉粥样硬化的形成和发展。
Objective: To investigate the effect of interleukin 1β(IL-1β) and tumor necrosis factorα(TNF-α) on expression of acyl-CoA cholesterol acyltransferase enzyme (ACAT) mRNA, and to discuss the mechanism of atherosclerosis. Method:Mice aortic vascular smooth muscle cells (VSMCs) were cultured with 100 ug/ml oxidized low density Lipoprotein for 72 hours to form foam cells(FCs). Both VSMCs and FCs derived from smooth muscle cells were incubated with IL-1β(10 ng/ml) or TNF-α (20 μg/L) for 24 hours, and ACAT mRNA was measured by semi-quantitative reverse transcription-polymerase chain reaction( RT-PCR) respectively. Result:①While VSMCs transformed into FCs , the expression of ACAT mRNA increased from 0.198 ± 0.068 to 0.425 ± 0.103 , with significant difference ( P < 0.05 ).②TNF-α had no significant influence on the expression of ACAT mRNA in VSMCs and FCs. ③IL-1β significantly increased the expression of ACAT mRNA of FCs derived from smooth muscle cells from 0.425 ± 0.103 to 0.650 ± 0.135 ( P < 0.05 ). Conclusion:IL-1β upregulates the expression of ACAT mRNA, stimulated cholesterol esterification and cholesterol deposition , and can participate in the initiation and progression of atherosclerosis.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2005年第7期414-416,共3页
Journal of Clinical Cardiology
基金
国家自然科学基金项目(No:30170378)
