摘要
目的探讨去甲状旁腺(PTG)模型鼠的制备方法,为PTG移植奠定实验基础。方法手术切取PTG,并行病理学检查。对术前和术后2、5、10、15和30d的大鼠血清钙和甲状旁腺激素(PTH)进行测定。结果8只鼠的PTG被成功切除,切除率为80%(8/10)。假手术组手术前、后血清钙和PTH水平无明显变化(P>0.05)。PTG切除组术后2、5、10、15和30d血清钙和PTH水平均明显低于术前,并明显低于同时相假手术组水平(P<0.01)。结论准确切取大鼠PTG,可成功制备去PTG模型鼠。术后10d可作为异体移植的适宜时间。
Objective To investigate the technique of establishing a model of aparathyroid rat which could be used in the study of parathyroid cells transplantation. Methods Parathyroid glands were surgically excised and (identified) pathologyically. Serum calcium and parathyroid hormone in rats before operation and on day 2,5,10,15 and 30 after operation were measured. Results Parathyroid glands were resected successfully in 8 rats, and the resection rate was 80% (8/10). No obvious changes of serum calcium and parathyroid hormone levels were found before and after operatiion in sham parathyroid gland excision group (P>0.05). However, statistically significant changes of those data were found perioperatively in parathyroid gland excision group (P<0.01). Conclusion The model of aparathyroid rat can be established successfully after parathyroid glands in rats are excised exactly. Parathyroid allotransplantation could be performed ten days after parathyriodectomy.
出处
《中国普外基础与临床杂志》
CAS
2005年第4期393-394,共2页
Chinese Journal of Bases and Clinics In General Surgery
关键词
甲状旁腺
手术
Parathyroid gland Operation
