摘要
目的:探讨清肺口服液含药血清对腺病毒3I、7b感染的人胚肺成纤维细胞之肿瘤坏死因子-α(TNF-α)蛋白表达的影响。方法:以3I、7b型腺病毒分别攻击体外培养的人胚肺成纤维细胞,制备清肺口服液含药血清,作用于该细胞,另设正常细胞组,病毒对照组,利巴韦林组,采用ELISA法检测不同组细胞上清液中TNF-α的含量,并进行比较。结果:病毒对照组较正常细胞组之TNF-α含量明显增多,清肺口服液含药血清组可显著降低3I、7b型腺病毒攻击后细胞TNF-α的浓度。结论:清肺口服液可低腺病毒感染所致人胚肺成纤维细胞TNF-α蛋白的高表达,这可能是其抗病毒作用的机制之一。
Objective: To study the effect of qingfel oral liquid contained serum on the tumor necrosis factor -α (TNF - α) protein expression of human embryonic lung flbroblast cells induced by adenovirus typ 3I、7b.Methods:the TNF-α concentration of ADV - infected human embryonic lung fibroblasl cells before and after adding the contained - herbs serum were determined with enzyme - linked immunosorbent assay (ELISA) Results: Adenovirus could increase the TNF -α concentration of human embryonic lung fibroblast cells, while qingfei oral liquid could decrease it. Conclusion: Qingfel oral liquid played an antiviral role by regulating some cytokine level.
出处
《中国中医药科技》
CAS
2005年第4期216-217,i002,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
国家自然科学基金项目No.30171171
