摘要
取４只家兔胫前骨膜进行成骨细胞分离培养，用３Ｈ－ＴｄＲ标记，然后回植于同一供体的皮下、耳软骨缺损处及挠骨缺损处。分别在２周和４周后处死动物，原位取材，用放射自显影方法观察种植细胞的转归。结果表明，种植的细胞在皮下转化为类骨组织；在软骨缺损处转化为软骨组织；而在骨缺损处则转化为典型的骨组织。提示用骨膜分离培养成骨细胞，回植体内，有可能用于骨缺损和软骨缺损的修复。
steoblasts were cultured and isolated from a piece of tibial
pettiosteum of four New-Zealandrabbits. After subeultured,these cells Were incubatd in vitro
with tritiated thvmidine for 36 hoursand then these labeled cells were implanted in the
subeutaneous layer of the defects of the auriclarcartilage and the radial bone, After 2 weeks
and 4 weeks respectively, these rabbits were killed andthe spoimens were obtained from the
site where the cells had been transplanted. The transformation ofthese cells was obeerved by
autoradiographlc method. The results indicated that nearly all of thecultured cells were labeled,
After 2 weeks, it was observed that many labeled osteoblasts were indifferent stages of
differentiation, some were beried by extracellular matrix and resembled coteocyte,others were
differentiated into chondrocvte-like cell.In addition, some labeled osteoblasts werecongregated
in the form of multinucleated coteoclast. After 4 weeks,in the subeutaneous layer thelabeled
coteoblasts were changed to osteoid tissue and in the defect of the auricular cartilage these
cellstransformed into chondritie tissue; moreover, those labeled osteoblsts which had been
implanted intothe radial defect had differentiated into typical bone tissue. The results of this
research indicated thatthe osteoblasts isolated from the pericateum if reimplanted to the same
donor might be poible to repairthe bone and cartilage defects.
出处
《中国修复重建外科杂志》
CAS
CSCD
1994年第3期168-171,T009,共5页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
成骨细胞
放射自显影
细胞培养
Periosteum
Osteoblasts
Cell culture
Osteogenesis
Osteoclasts
Autoradiographic study
