摘要
目的观察甲基强的松龙对大鼠颅脑损伤后血脑组织中TNF含量的影响,并探讨其作用机制. 方法将55只SD大鼠随机分为3组,采用骨窗形成后硬膜外打击法造成鼠脑挫裂伤模型,正常组5只,麻醉行开颅手术,不作头颅打击;治疗组大鼠致伤后即刻腹腔内注射30 mg/kg甲基强的松龙,对照组则即刻腹腔内注射30 mg/kg生理盐水.对照组和治疗组大鼠分别在伤后6 h、24 h、48 h、72 h、96 h时间点断头取脑,对大鼠脑外伤后脑组织中TNF含量进行检测. 结果大鼠脑皮质中的TNF活性在伤后6 h后较对照组升高显著(P<0.01),48 h达高峰,96 h降至基础水平.甲基强的松龙治疗组在伤后6～48 h TNF活性较损伤组明显降低(P<0.05). 结论颅脑损伤后,受损脑组织中TNF含量升高,甲基强的松龙可通过抑制损伤后TNF活性,起到保护创伤神经元的作用.
Objective The experiment was conducted to observe the role of methylprednisolone according to the change of TNF level in brain trauma tissue of rat. Methods 55 rats were made by frontoparietal bone window plasty with extradural hitting, and were divided into three groups: treated group (treated with methylprednisolone 30 mg/kg after hitting), contral group (treated with physiologic saline after hitting) and normal group (only expose extradural without hitting and injection). The levels of TNF were measured at different points of time after hitting. Results TNF level in rat cerebral cortes was higher than that in the contrasting group post-traumatic 6 hour, and reach to peak post-traumatic 48 hour and dropped to the basic level post-traumatic 72 hour. The level of TNF in Methylprednisolone treatment group was obviously lower than that in the injured group post-traumatic 6～48 hour. Conclutions Methylprednisolone is effecttive on head injury.
出处
《现代医院》
2005年第8期19-20,共2页
Modern Hospitals
