摘要
目的探讨黄芪活性成分黄芪甲甙对病毒性心肌炎(VM)小鼠心肌中组织蛋白酶B(cathepsinB,CB)mRNA表达的影响。方法Balb/c小鼠100只随机分为6组。非感染小鼠腹腔无菌注射病毒培养液,分为正常对照组(A组10只,以羧甲基纤维素钠0.1mL灌胃7d)、9%黄芪甲甙对照组(B组10只,9%黄芪甲甙0.1mL灌胃7d);余80只小鼠以柯萨奇病毒(CVB3)腹腔无菌注射制作VM模型,VM小鼠随机分为VM对照组和1%、3%、9%黄芪甲甙干预VM组,分别以生理盐水、1%、3%、9%黄芪甲甙0.1mL灌胃7d(分别为C、D、E、F组,每组20只)。14d后处死全部小鼠并取其心脏。采用RT-PCR半定量检测心肌CBmRNA表达水平。结果F组小鼠较C组死亡率明显降低[10%(2/20)vs45%(9/20),χ2=6.14P<0.05],B组无小鼠死亡;与C组比较CBmRNA表达水平及心肌病变积分在F组显著下降,而1%、3%黄芪甲甙对VM小鼠CBmRNA表达及心肌病变积分无明显影响。结论黄芪甲甙可能通过抑制CB表达,对Balb/c小鼠CVB3心肌炎具有良好的治疗作用。
Objective To investigate the effect of astragaloside on myocardial cathepsin B(CB)mRNA expression, one of the active components of astragalus membranaceus, in coxsackievirus B_3(CVB_3)inducing murine myocarditis model.Methods One hundred Balb/c mice were randomly divided into 6 groups.Group A [ n =10,treated with carboxymethycellulose(CMC) 0.1 mL,intragastric administration (ig) for 1 week] was served as normal control and group B[ n =10,treated with 9% astragaloside 0.1 mL, ig, for 1 week]as treatment control.Eighty infected animals treated with CMC and 1%,3%,9% astragaloside 0.1 mL,ig,for 1 week( n =20,in each group)were served as group C,D,E and F. The mice were killed and their hearts were removed after the 14th day. The expression levels of CB mRNA in the myocardium were examined by RT-PCR.Results The mortality was significantly reduced in 9% astragaloside treated infected animals that was 10%(2/20) vs 45%(9/20)in group C, χ 2=6.14 P <0.05. In uninfected animals, 9% astragaloside did not cause any death. The expression levels of CB mRNA and myocardial histopathologic scores were significantly lower in group F than those in group C. However, the CB expression and myocardial histopathologic scores were not affected by 1% and 3% astragaloside.Conclusions Astragaloside might play a pivotal role in curing the heart injure in Balb/c mice with CVB_3 myocarditis by suppressing excessive expression of myocardial cathepsin B.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第7期622-624,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30271665)
