摘要
以MS,B5,N6,NN,6,7 V,WP为基本培养基,分析了不同类型培养基对甘草愈伤组织生长及黄酮类化合物生物合成的影响,并考察了培养基中添加的激素种类和浓度以及培养基酸碱度的作用。结果表明:在6种基本培养基中,以B5培养基最利于生物量的积累,异甘草素含量最高WP培养基最利于甘草素的合成,其次是6,7 V培养基,以N6培养基最差;当培养基中添加0 1mg LNAA时,甘草素含量最高,达57 24μg g,当培养基中添加1 0mg LNAA时,异甘草素含量最高,达36 45μg g;pH值为6时,甘草愈伤组织生物量积累最高,同时对黄酮类化合物甘草苷和甘草素的生物合成也最为有利,尤其是甘草苷,积累量最高,达46 88μg g,比其它pH值处理高152 8%～245 5%。
<Abstrcat> MS, B5, N6, NN, 6,7-V and WP were used as basal media to study their effects on callus growth and flavonoids production of Glycurrhiza uralensis, and the effects of phytohormones and pH of media were investigated. The results show that among the tested basal media, B5 was the best for callus growth. WP was most suitable for liquiritigenin production, and maximum isoliquiritigenin was observed in B5. Liquiritigenin content was the highest with 57.24 μg/g when 0.1 mg/L NAA was added to media, and isoliquiritigenin accumulation was the highest with 36.45 μg/g when NAA concentration was (1.0 mg/L). The optimum pH of media for biomass accumulation of callus and biosynthesis of liquiritin and liquiritigenin was 6.0, and especially liquiritin content was the highest with 46.88 μg/g and higher than others by 152.8%～245.5%.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2005年第3期289-292,295,共5页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(39470849)
