摘要
本文报道了2,4-D、BA和GA_3对丹参悬浮培养细胞生长和酚酸类化合物含量的影响。在继代培养基(MS+2,4-D 1 mg/L+KT 0.1 mg/L)中,培养第12天细胞生长和迷迭香酸含量达到最大值,而紫草酸B的含量在培养的第16天达到最大值,证实产物的形成属于生长依赖型。在相同浓度(1 mg/L)时,2,4-D促进细胞生长和抑制紫草酸B的形成,对迷迭香酸作用不明显;GA_3抑制细胞生长和促进二种化合物的形成;BA的作用则介于两者之间。浓度实验发现,BA的最适浓度为3 mg/L,GA_3为1 mg/L,而最适加入时间分别为培养中期(第8天)和早期(第0天)。当以MS+BA 3 mg/L为基本培养基而在不同培养时间加GA_3(1mg/L)时,细胞生长和酚酸类化合物形成提前到第6天达到最大值,迷迭香酸含量虽未能超过单加GA_3的数值,但紫草酸B则超过单加GA_3的数值,表明两种激素有一定的协同作用。
This paper deals with the effects of 2,4-D,BA and GA_3 on the growth and content of two depsides (rosmarinic acid and lithospermic acid B) in suspension cells of Salvia miltiorrhiza.The results showed cell growth and rosmarinic acid content reached the maximum on the 12th day and lithospermic acid B content on the 16th day after incubating cells in the subculture medium MS + 2,4-D 1 mg,/L + KT 0.1 mg,/L.This cell line was a growth-product-associated.With the same concentration (1 mg/ L),2,4-D stimulated the cell growth but prohibited the formation of lithospermie acid B;GA_3 inhabited the cell gowth but stimulat- ed the formation of two depsides;The effects of BA is between 2,4-D and GA_3.The concentration optimum of phytohornones tested displayed 3 mg/L for BA and 1 mg/L for GA_3.For the optimum time of adding BA and GA_3 was in med-term (the 8th day) and early term (at the beginning) of culture period respectively.The synergiatic function of BA and GA_3 on the depside formation was also showed that adding GA_3 (1 mg/L) was favour for the formation of lithospermic acid B of the suspension cell cultured in the medium containing BA 3 mg/L.The suitable time of adding GA_3 was the 6the day after incubating cells in the medium of MS + BA 3mg/L.
出处
《中药材》
CAS
CSCD
2000年第1期1-4,共4页
Journal of Chinese Medicinal Materials
基金
上海市卫生局和上海市教委资助项目
关键词
丹参
悬浮培养细胞
迷迭香酸
紫草酸B
Salvia miltiorrhiza
Suspension cells
Rosmarinic acid
Lithospermic acid B
