摘要
４０天龄鹌鹑连续１０天肌注卡那霉素造成耳中毒后，分别于停药后１天或１、２、３、４和６周进行听神经动作电位测试及扫描电镜观察。发现停药后１天动物毛细胞大量破坏，听功能严重受损，同时还出现了新生毛细胞。随着时间推移，新生毛细胞逐渐增多，听功能也随之改善，直至停药后第６周耳蜗形态基本恢复正常时，高频听阈仍明显高于对照组。本实验结果表明，鹌鹑听毛细胞受损后几乎可完全再生，而且再生毛细胞具有感音功能，但功能改善明显迟于形态恢复。
Forty-day old quails were given kanamycin(KM) at a dose of 400mg/kg per day for ten days.Evoked potential thresholds induced by both click and tone burst were obtained at 1 day or 1,2,3,4 and 6 weeks respectively after the last injection, and compared with the thresholds of age-matched con-trol animals.The cochlea of the animals was examined by scanning electron microscopy(SEM). The re-sults showed that the application of KM for 10 days produced massive destruction of the basilar papilla at 1 day posttreatment.The dam aged region began at the base and extended apically to the position about 50～70% of the total distance along the papilla,with most severe damage in the basal end.The auditory thresholds measured at the same time revealed a significant elevation at 2 and 4 kHz. However,the number of hair cells in the damaged region recovered rapidly with time,and so did the function. By the end of 3 weeks postinjection,almost all the hair cells reappeared in the pathologic region and the function improved further,with nearly nonnal thresholds at all frequencies but 4kHz,Six weeks after KM treatment,the basilar papilla showed a nearly normal appearance, but there was no further function-al improvement at 4kHz, with the threshold shift by 17.5 dB(P<0.01).The results of the present study demonstrated that structural recoverv was faster than functional one.
出处
《中华耳鼻咽喉科杂志》
CSCD
1994年第2期85-88,T011,共5页
Chinese Journal of Otorhinolaryngology
